PTTG1/2/3 rabbit pAb Antibody

SKU: orb766157

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Description

Rabbit polyclonal antibody to PTTG1/2/3

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human PTTG1. AA range:111-160
Molecular Weight	30
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-PTTG1 antibody, anti-EAP1 antibody, anti-PTTG antibody, anti-TUTR1 antibody, anti-Securin antibody, anti-Esp1-associated protein antibody, anti-Pituitary tumor-transforming gene 1 protein antibody, anti-Tumor-transforming protein 1 antibody, anti-hPTTG antibody

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Western Blot analysis of various cells using PTTG1/2/3 Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of HUVEC cells, using PTTG1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, Jurkat, and 293 cells, using PTTG1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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