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RAB11A Antibody

SKU: orb1787868

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology, Signal Transduction

Images & Validation

Tested ApplicationsIHC-P, WB
Dilution RangeWB: 1:4000, IHC-P: 1:25
ReactivityHuman, Mouse, Rat
Predicted ReactivityPorcine, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1,k
ImmunogenThis RAB11A antibody is generated from a mouse immunized with a purified recombinant protein of human RAB11A. Antigen Region: 1-216 aa.
TargetRAB11A (HGNC:9760)
Molecular Weight24394 Da
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Ras-related protein Rab-11A, Rab-11, YL8, RAB11A, RAB11

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RAB11A Antibody

All lanes: Anti-RAB11A Antibody at1:4000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: PC-12 whole cell lysate. Lane 6: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RAB11A Antibody

Staining RAB11A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

RAB11A Antibody (orb1787868)

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