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Rabbit anti-Bcl11b Antibody
SKU: orb1530280
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:5,000 - 1:15,000; IP - 1 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 500 and 550 |
| Target | Bcl11b |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−hRIT1-alpha; ZNF856B; zinc finger protein hRit1 alpha; RIT1; radiation-induced tumor suppressor gene 1 protein; IDDFSTA; hRit1; CTIP-2; CTIP2; COUP-TF-interacting protein 2; BCL11B/TRDC fusion; ATL1-gamma; IMD49; BCL-11B; ATL1-alpha; ATL1-beta; ATL1-delta; B-cell CLL/lymphoma 11B; ATL1; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B
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Detection of human Bcl11b by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-Bcl11b antibody orb1530280 at 0.066 µg/ml.
Detection of human Bcl11b by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1, 000 (1µg/ml).
Detection of human Bcl11b by WB and IP.Samples: Nuclear extract from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody used at 0.1 µg/ml (A) and 0.5 µg/ml (B) for WB and at 1 and 10 µg/mg lysate for IP (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_612808.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-Bcl11b Antibody (orb1530280)
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