Rabbit Beta-2-adaptin Antibody

SKU: orb1522465

Description

Beta-2-adaptin Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Tested Applications	IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 575 and 625
Target	Beta-2-adaptin
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta); adaptor protein complex AP-2 subunit beta; adaptor related protein complex 2 beta 1 subunit; adaptor-related protein complex 2 subunit beta; ADTB2; AP105B; AP-2 complex subunit beta; AP2-BETA; beta-2-adaptin; beta-adaptin; CLAPB1; clathrin assembly protein complex 2 beta large chain; clathrin-associated/assembly/adaptor protein, large, beta 1; plasma membrane adaptor HA2/AP2 adaptin beta subunit; testicular tissue protein Li 22

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Detection of mouse Beta-2-adaptin by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CH27, BW5147.3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Beta-2-adaptin by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEK293T, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Beta-2-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Beta-2-adaptin antibody (orb1522465) used for IP at 6 µg per reaction. Beta-2-adaptin was also immunoprecipitated by a previous lot of this antibody (orb1522465) and a second antibody against a different epitope of Beta-2-adaptin.

Quick Database Links

Gene Symbol

Beta-2-adaptin

UniProt

P63010

RefSeq (Protein):NP_001273.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001273.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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