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Rabbit BRD3 Antibody

SKU: orb1526750

Description

BRD3 Antibody

Images & Validation

Tested ApplicationsIHC, IP, WB
Dilution RangeWB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ReactivityHuman
Application Notes
Format: Whole IgG

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenBetween 676 and C-terminus
TargetBRD3
PurificationAntigen Affinity Purified
ConjugationUnconjugated

Storage & Handling

Storage2 - 8°C
Form/AppearanceLiquid
Buffer/PreservativesTris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration200 µg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

bromodomain-containing protein 3; bromodomain-containing protein 3 short isoform; ORFX; RING3L; RING3-like protein

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Rabbit BRD3 Antibody

Detection of human BRD3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit BRD3 Antibody

Detection of human BRD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for IP at 6 µg per reaction.

Rabbit BRD3 Antibody

Detection of human BRD3 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma.Antibody: Affinity purified rabbit anti-BRD3 (orb1526750) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

Rabbit BRD3 Antibody (orb1526750)

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Available Sizes

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10 μl
¥ 3,120.00
100 μl
¥ 8,190.00
DispatchUsually dispatched within 5-10 working days
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