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Rabbit BRD3 Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 676 and C-terminus |
| Target | BRD3 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−BRD3 Rabbit Polyclonal Antibody [orb575083]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
Human
Rabbit
Polyclonal
Unconjugated
100 μlBRD3 Rabbit Polyclonal Antibody [orb625175]
ELISA, IHC, IP, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μg, 100 μg

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Detection of human BRD3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BRD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD3 antibody orb1526750 used for IP at 6 µg per reaction.

Detection of human BRD3 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma.Antibody: Affinity purified rabbit anti-BRD3 (orb1526750) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_031397.1 |
|---|
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Protocol Information
Rabbit BRD3 Antibody (orb1526750)
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