Rabbit anti-BRG1/SMARCA4 Antibody

SKU: orb1529587

Description

BRG1/SMARCA4 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 75 and 125
Target	BRG1/SMARCA4
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; transcription activator BRG1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; sucrose nonfermenting-like 4; mitotic growth and transcription activator; SWI2; SNF2-like 4; BRG1; MRD16; nuclear protein GRB1; BAF190; brahma protein-like 1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; BAF190A

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Detection of human BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from Jurkat, K-562, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.

Detection of mouse BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.

Detection of mouse BRG1/SMARCA4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human BRG1/SMARCA4 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody.

Quick Database Links

Gene Symbol

BRG1/SMARCA4

UniProt

P51532

RefSeq (Protein):NP_003063.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003063.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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