Rabbit anti-CD48 Recombinant Monoclonal Antibody

SKU: orb1784565

Description

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IHC, WB
Dilution range	WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR275L
Immunogen	Residues 23-216 (ECD)
Target	CD48

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	250 µg/ml
Disclaimer	For research use only

Alternative Names

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BLAST-1; HM48-1; SLAMF2; SLAM family member 2; signaling lymphocytic activation molecule 2; Sgp-60; MRC OX-45 surface antigen; MEM-102; AW610730; AI449234; BLAST1; BLAST; BCM1 surface antigen; Bcm-1; BCM1; CD antigen CD48; CD48 antigen

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Rabbit anti-CD48 Recombinant Monoclonal Antibody

Detection of mouse CD48 by western blot. Samples: Whole cell lysate (25 µg) from 70Z/3, WEHI-231, CTLL-2, A20, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD48 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD48 by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CD48

UniProt

P18181

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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