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Rabbit anti-CD73 Recombinant Monoclonal Antibody
SKU: orb1519866
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution range | WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLR054F |
| Immunogen | Residues 27-552 (ECD) |
| Target | CD73 |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−eN; NTE; NT5; CALJA; NT; Purine 5-Prime-Nucleotidase; eNT; E5NT; CD antigen CD73; 5'-nucleotidase; 5'-NT; CD73; ecto-5'-nucleotidase
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Detection of human CD73 by western blot. Samples: Whole cell lysate (50 µg) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Detection of human CD73 by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human CD73 by immunocytochemistry. Sample: FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002517.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Rabbit anti-CD73 Recombinant Monoclonal Antibody (orb1519866)
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