Rabbit CD8 alpha Recombinant Monoclonal Antibody

SKU: orb1519887

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Description

CD8 alpha Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC, WB
Dilution Range	Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR044F
Immunogen	Between 185 and C-terminus
Target	CD8 alpha
Purity	Recombinant antibody was purified from cell culture supernatant
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	250 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD antigen CD8a; CD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Rabbit CD8 alpha Recombinant Monoclonal Antibody

Detection of human CD8 alpha by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG). Substrate: DAB

Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human melanoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Detection of human CD8 alpha (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Detection of human CD8 alpha by western blot. Samples: Whole cell lysate (50 µg) from CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Recombinant monoclonal antibodies to CD3e and CD4

Quick Database Links

Gene Symbol

CD8 alpha

UniProt

P01732

RefSeq (Protein):NP_741969.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_741969.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence