Rabbit CD86 Recombinant Monoclonal Antibody

SKU: orb1710829

Description

CD86 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IP, WB
Dilution Range	WB: 1:1,000, IP: 6 µl/1 mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR255L
Immunogen	Residues 24-245 (ECD)
Target	CD86
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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activation B7-2 antigen; B7; B7.2; B70; B7-2; CD antigen CD86; Cd28l2; CLS1; early T cell costimulatory molecule-1; Early T-cell costimulatory molecule 1; ETC-1; Ly58; Ly-58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2

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Rabbit CD86 Recombinant Monoclonal Antibody

Detection of mouse CD86 by western blot. Samples: Whole cell lysate (10 µg) from A20, CH27, WEHI-231, J774A1, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710829) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD86 (shaded) in CH27 cells by flow cytometry. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710829) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse CD86 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from CH27 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710829) used for IP at 6 µl/mg lysate.

Detection of mouse CD86 by immunocytochemistry. Sample: FFPE section of mouse CH27 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710829). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD86 by immunocytochemistry. Sample: FFPE section of mouse J774A1 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710829). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CD86

UniProt

P42082

RefSeq (Protein):NP_062261.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_062261.3

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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