Rabbit DLST Antibody

SKU: orb1523236

Description

DLST Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 50 and 100
Target	DLST
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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2-oxoglutarate dehydrogenase complex component E2; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; DLTS; E2K; OGDC-E2

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Detection of human and mouse DLST by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-DLST antibody orb1523236 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DLST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-DLST antibody orb1523236 used for IP at 6 µg per reaction.

Detection of human DLST by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DLST (Cat. No. orb1523236) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human DLST by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLST (Cat. No. orb1523236) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

DLST

UniProt

P36957

RefSeq (Protein):NP_001924.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001924.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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