Rabbit EDD1 Antibody

SKU: orb1529971

Description

EDD1 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 625 and 675
Target	EDD1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DD5; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; EDD; EDD1; HECT-type E3 ubiquitin transferase UBR5; hHYD; HYD; hyperplastic discs protein homolog; progestin-induced protein

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Detection of human EDD1 by western blot.Samples: Whole cell lysate from HEK293T, Jurkat, and LNCaP cells. Antibody: Affinity purified Rabbit anti-EDD1 antibody orb1529971 at 0.4 µg/ml.

Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-EDD1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-EDD1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human EDD1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody.

Quick Database Links

Gene Symbol

EDD1

UniProt

O95071

RefSeq (Protein):NP_056986.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_056986.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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