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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 208 and C-terminus |
| Target | eIF3J/EIF3S1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−eIF3 p35; eIF-3-alpha; eIF3-alpha; eIF3j; eIF3-p35; EIF3S1; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
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100 μl, 10 μl- Item 1 of 1
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Detection of human eIF3J/EIF3S1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3J/EIF3S1 antibody orb1527865 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF3J/EIF3S1 was also immunoprecipitated by rabbit anti-eIF3J/EIF3S1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human and mouse eIF3J/EIF3S1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 (Cat. No. orb1527865) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003749.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit eIF3J/EIF3S1 Antibody (orb1527865)
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