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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:2,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 546 and C-terminus |
| Target | ENH/PDLIM5 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ENH; ENH1; enigma homolog; enigma-like LIM domain protein; enigma-like PDZ and LIM domains protein; L9; LIM; PDZ and LIM domain protein 5
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Detection of human and mouse ENH/PDLIM5 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody orb1527939 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human ENH/PDLIM5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody orb1527939 used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006448.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit ENH/PDLIM5 Antibody (orb1527939)
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