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Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody
SKU: orb1519833
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:1000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLR068G |
| Immunogen | Between 191 and C-terminus |
| Target | GITR/TNFRSF18 |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−GITR-D; tumor necrosis factor receptor superfamily member 18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; GITR; CD357; CD antigen CD357; AITR; TNF receptor superfamily activation-inducible protein
Similar Products
−- Item 1 of 3
Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [orb1519832]
FC, ICC, IHC, IP, WB
Human
Rabbit
Recombinant
Unconjugated
10 μl
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Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 µg) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody.
Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004186.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody (orb1519833)
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