Rabbit GLI1 Recombinant Monoclonal Antibody

SKU: orb1710827

Description

GLI1 Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IP, WB
Dilution Range	WB: 1:1,000, IP: 20 µl/1 mg lysate
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR256L
Immunogen	Between 1056 and C-terminus
Target	GLI1
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	100 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GLI; GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; PAPA8; zinc finger protein GLI1

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Rabbit GLI1 Recombinant Monoclonal Antibody

Detection of human GLI1 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, PC-3, RMS-13, MCF-7, and TOV-112D cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710827) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human GLI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RMS-13 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710827) used for IP at 20 µl/mg lysate.

Quick Database Links

Gene Symbol

GLI1

UniProt

P08151

RefSeq (Protein):NP_005260.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005260.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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