Rabbit Hakai Antibody

SKU: orb1525683

Description

Hakai Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP
Dilution range	WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	Hakai
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; casitas B-lineage lymphoma-transforming sequence-like protein 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; c-Cbl-like protein 1; E3 ubiquitin-protein ligase Hakai; E-cadherin binding protein E7; HAKAI; RING finger protein 188; RING-type E3 ubiquitin transferase Hakai; RNF188

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Detection of human Hakai by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Hakai antibody orb1525683 used for IP at 3 µg/mg lysate.

Detection of human Hakai by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Hakai (Cat. No. orb1525683) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Hakai

UniProt

Q75N03

RefSeq (Protein):NP_079090.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_079090.2

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Protocol Information

IHC

Immunohistochemistry

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Immunoprecipitation

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