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Rabbit IgM Recombinant Monoclonal Antibody
SKU: orb1806431
Description
Images & Validation
−Item 1 of 8
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 20 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR294L |
| Immunogen | Human IgM |
| Target | IgM |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of human IgM by western blot. Samples: Whole cell lysate (10 µg) from Daudi, MOLT-4, Raji, HEK293T, and Ramos cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse IgM by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, WEHI-231, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human IgM by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human IgM by immunohistochemistry. Sample: FFPE section of B-cell lymphoma. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human IgM (shaded) in Raji cells by flow cytometry. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human IgM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431) used for IP at 20 µl/mg lysate.
Detection of human IgM by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse IgM by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-IgM recombinant monoclonal antibody (orb1806431). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
Gene Symbol
IgM
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit IgM Recombinant Monoclonal Antibody (orb1806431)
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