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Rabbit JDP2 Recombinant Monoclonal Antibody
SKU: orb1733245
Description
Images & Validation
−Item 1 of 9
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR246L |
| Immunogen | Between 50 and 100 |
| Target | JDP2 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 400 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−jun dimerization protein 2; JUNDM2; progesterone receptor co-activator
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Rabbit JDP2 Recombinant Monoclonal Antibody [orb1733244]
FC, ICC, IHC, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μg (BSA-free)
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Detection of mouse JDP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and RenCa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by western blot. Samples: Whole cell lysate (25 µg) from MCF-7, HeLa, HaCaT, and DU145 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunohistochemistry. Sample: FFPE section of mouse RenCa tumor. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunocytochemistry. Sample: FFPE section of human FaDu cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_569736.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
Rabbit JDP2 Recombinant Monoclonal Antibody (orb1733245)
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