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Rabbit JMJD6 Recombinant Monoclonal Antibody
SKU: orb1710831
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR254L |
| Immunogen | Between 125 and 175 |
| Target | JMJD6 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−arginine demethylase and lysine hydroxylase; bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; histone arginine demethylase JMJD6; jmjC domain-containing protein 6; jumonji domain containing 6; jumonji domain-containing protein 6; lysyl-hydroxylase JMJD6; peptide-lysine 5-dioxygenase JMJD6; phosphatidylserine receptor; Protein PTDSR; PSR; PTDSR; PTDSR1
Quality Guarantee
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Detection of mouse JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, NIH 3T3, CH27, ND7/23, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, RKO, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JMJD6 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 by immunocytochemistry. Sample: FFPE section of human A549 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunocytochemistry. Sample: FFPE section of mouse ND7/23 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_055982.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
Rabbit JMJD6 Recombinant Monoclonal Antibody (orb1710831)
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