Rabbit Ki-67 Recombinant Monoclonal Antibody

SKU: orb1519949

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Description

Ki-67 Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IF, IHC
Dilution Range	Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. ICC-IF 1:100 - 1:500. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR021E
Immunogen	Between 1200 and 1250
Target	Ki-67
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	50 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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antigen identified by monoclonal antibody Ki-67; antigen Ki67; Antigen KI-67; KIA; MIB-; MIB-1; PPP1R105; proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105

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Rabbit Ki-67 Recombinant Monoclonal Antibody

Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (orb1519949) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.

Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

Ki-67

UniProt

P46013

RefSeq (Protein):NP_002408.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002408.3

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Protocol Information

IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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