Rabbit Leo1 Antibody

SKU: orb1530587

Description

Leo1 Antibody

Images & Validation

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Item 1 of 5

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 550 and 600
Target	Leo1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Leo1 Paf1/RNA polymerase II complex component; Leo1, Paf1/RNA polymerase II complex component, homolog; RDL; replicative senescence down-regulated leo1-like protein; RNA polymerase-associated protein LEO1

Quality Guarantee

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Detection of human Leo1 by western blot.Samples: Whole cell lysate from HeLa, MCF-7, and Jurkat cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530587 at 0.04 µg/ml.

Detection of mouse Leo1 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530587 at 0.04 µg/ml.

Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).

Detection of human Leo1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).

Detection of human Leo1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Leo1 antibody.

Quick Database Links

Gene Symbol

Leo1

UniProt

Q8WVC0

RefSeq (Protein):NP_620147.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_620147.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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