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Rabbit MSH2 Recombinant Monoclonal Antibody
SKU: orb1519706
Description
Research Area
Cancer Biology, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR116H |
| Immunogen | Between 625 and 675 |
| Target | MSH2 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2
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Detection of human MSH2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, K-562, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MSH2 recombinant monoclonal antibody (orb1519706) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human MSH2 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-MSH2 recombinant monoclonal antibody (orb1519706). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human MSH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MSH2 recombinant monoclonal antibody (orb1519706) used for IP at 20 µl/mg lysate.
Detection of human MSH2 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-MSH2 recombinant monoclonal antibody (orb1519706). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000242.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit MSH2 Recombinant Monoclonal Antibody (orb1519706)
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