Rabbit NCBP1/CBP80 Antibody

SKU: orb1527777

Description

NCBP1/CBP80 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Xenopus
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	NCBP1/CBP80
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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80 kDa nuclear cap-binding protein; CBP80; NCBP; NCBP 80 kDa subunit; nuclear cap binding protein subunit 1, 80kDa; nuclear cap-binding protein subunit 1; Sto1

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Detection of human NCBP1/CBP80 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-NCBP1/CBP80 antibody orb1527777 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NCBP1/CBP80 was also immunoprecipitated by rabbit anti-NCBP1/CBP80 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Detection of human NCBP1/CBP80 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NCBP1/CBP80 (Cat. No. orb1527777) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NCBP1/CBP80

UniProt

Q09161

RefSeq (Protein):NP_002477.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002477.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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