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Rabbit P-Selectin/CD62P Recombinant Monoclonal Antibody
SKU: orb1784576
Featured
Description
Research Area
Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:100. Western Blot (WB) 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR267L |
| Immunogen | Residues 42-771 (ECD) |
| Target | P-Selectin/CD62P |
| Purity | Recombinant antibody was purified from cell culture supernatant |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD antigen CD62P;CD62;CD62 antigen-like family member P;CD62P;GMP140;GMP-140;granule membrane protein 140;granule membrane protein 140kDa;granulocyte membrane protein;GRMP;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;platelet alpha-granule membrane protein;PSEL;P-selectin;selectin P (granule membrane protein 140kDa, antigen CD62)
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Rabbit P-Selectin/CD62P Recombinant Monoclonal Antibody [orb1784574]
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Recombinant
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Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human P-Selectin/CD62P (red) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002996.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
Rabbit P-Selectin/CD62P Recombinant Monoclonal Antibody (orb1784576)
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