Rabbit PLRG1 Antibody

SKU: orb1527520

This product can be custom made

Description

PLRG1 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 - 1:500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 464 and C-terminus
Target	PLRG1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cwc1; pleiotropic regulator 1; pleiotropic regulator 1 (PRL1 homolog, Arabidopsis); PRL1; PRP46; PRPF46; TANGO4; transport and golgi organization 4 homolog

Quality Guarantee

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Detection of human and mouse PLRG1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PLRG1 antibody orb1527520 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PLRG1 was also immunoprecipitated by rabbit anti-PLRG1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

Detection of human PLRG1 by immunohistochemistry.Sample: FFPE sections of human prostate carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-PLRG1 (Cat. No. orb1527520) used at a dilution of 1: 200 (1µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100

Quick Database Links

Gene Symbol

PLRG1

UniProt

O43660

RefSeq (Protein):NP_002660.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002660.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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