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Rabbit SMC1 Recombinant Monoclonal Antibody
SKU: orb1519958
Description
Research Area
Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 9
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BL-205-2G8 |
| Immunogen | Between 1183 and C-terminus |
| Target | SMC1 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 50 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A
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Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of human SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Jurkat, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse SMC1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human SMC1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used for IP at 20 µl/mg lysate.
Detection of human SMC1 by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006297.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit SMC1 Recombinant Monoclonal Antibody (orb1519958)
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