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Rabbit Tyrosine Hydroxylase Recombinant Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR155J |
| Immunogen | Between 478 and C-terminus |
| Target | Tyrosine Hydroxylase |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Phosphate Buffered Saline (PBS) containing 0.2% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of mouse Tyrosine Hydroxylase by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, ND7/23, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody (orb1519609) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000351.2 |
|---|
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Protocol Information
Rabbit Tyrosine Hydroxylase Recombinant Monoclonal Antibody (orb1519609)
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