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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 257 and C-terminus |
| Target | UFD1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−UB fusion protein 1; ubiquitin fusion degradation 1 like; Ubiquitin fusion degradation protein 1; ubiquitin fusion degradation protein 1 homolog; ubiquitin recognition factor in ER-associated degradation protein 1; UFD1L
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Detection of human UFD1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-UFD1 antibody orb1527632 used for WB at 0.04 µg/m. Detection: Chemiluminescence with exposure time of 3 seconds.
Detection of human UFD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UFD1 antibody orb1527632 used for IP at 3 µg/mg lysate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005650.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit UFD1 Antibody (orb1527632)
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