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Rabbit IgG (H&L) Antibody Texas Red Conjugated
SKU: orb347709
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, FC, FLISA, IF, WB |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Rabbit |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Rabbit IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
| Conjugation | Texas Red |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat Anti-Rabbit IgG Antibody Texas Red Conjugated, Goat Anti Rabbit IgG Texas Red Conjugated Antibody
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Dot Blot showing the detection of Rabbit IgG. A three-fold serial dilution of Rabbit IgG starting at 200 ng was spotted onto 0.45 µm nitrocellulose. After blocking in 5% Blotto (p/n orb348624) 1 Hour at 20°C, Anti-Rabbit IgG (H&L) (GOAT) Antibody Texas Red Conjugated (p/n orb347709) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637).
Western blot of Texas Red™ Conjugated Goat Anti-Rabbit IgG secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
Rabbit IgG (H&L) Antibody Texas Red Conjugated (orb347709)
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