Rat Alanine Aminotransferase (ALT) ELISA Kit

SKU: orb776547

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase(ALT).

Research Area

Gastroenterology & Hepatology, Infectious Disease & Virology, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 50 U/L. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.79-50 U/L
Sensitivity	0.26 U/L
Concentration	50 U/L

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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GPT ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, AAT1 ELISA Kit, GPT1 ELISA Kit, Serum Glutamic Pyruvic Transaminase ELISA Kit, Alanine Transaminase ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit

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Rat Alanine Aminotransferase (ALT) ELISA Kit

Quick Database Links

UniProt

P25409

UniProt Details

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No UniProt data available

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Product Information

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Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Rat (1)

Piotr Franciszek Kurzyna et al. Cannabidiolic acid as a modulator of lipid metabolism in the liver of rats with metabolic-associated steatotic liver disease Sci Rep, (2026)

PubMed: 41792203

Applications

ELISA

Reactivity

Rat