Rat Arginine Vasopressin (AVP) ELISA Kit

SKU: orb782678

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin (AVP).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AVP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AVP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AVP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.116 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ARVP ELISA Kit, AVP ELISA Kit, VP ELISA Kit, Arginine Vasopressin ELISA Kit, Arg-Vasopressin ELISA Kit, Antidiuretic Hormone ELISA Kit, Diabetes Insipidus ELISA Kit, Neurohypophyseal ELISA Kit, Argipressin ELISA Kit, Vasopressin ELISA Kit

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Rat Arginine Vasopressin (AVP) ELISA Kit

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UniProt

P01186

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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