Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit

SKU: orb775460

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase IV(DPP4).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.63-40 ng/mL
Sensitivity	0.245 ng/mL
Concentration	40 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD26 ELISA Kit, ADA ELISA Kit, DPPIV ELISA Kit, DPP-IV ELISA Kit, ADABP ELISA Kit, ADCP2 ELISA Kit, TP103 ELISA Kit, Adenosine Deaminase Complexing Protein 2 ELISA Kit, T-cell activation antigen CD26 ELISA Kit

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Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit

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UniProt

P14740

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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