Rat Glycoprotein 39, Cartilage (GP39) ELISA Kit

SKU: orb780780

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 39, Cartilage(GP39).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GP39. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GP39. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GP39, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GP39 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.26 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CHI3L1 ELISA Kit, CHI3-L1 ELISA Kit, YKL40 ELISA Kit, Chondrex ELISA Kit, Chitinase 3-Like 1 ELISA Kit, Chondrocyte Protein YKL40 ELISA Kit, 39 kDa synovial protein ELISA Kit

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Rat Glycoprotein 39, Cartilage (GP39) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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