Rat Growth Regulated Oncogene Alpha (GROa/CXCL1) ELISA Kit

SKU: orb775102

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3(NAP3).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.7 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CXCL1 ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, GRO-A ELISA Kit, MGSA-A ELISA Kit, NAP3 ELISA Kit, SCYB1 ELISA Kit, Chemokine C-X-C-Motif Ligand 1 ELISA Kit, Melanoma Growth Stimulating Activity Alpha ELISA Kit, Fibroblast Secretory Protein ELISA Kit

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Rat Growth Regulated Oncogene Alpha (GROa/CXCL1) ELISA Kit

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UniProt

P14095

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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