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Rat IgM Antibody Peroxidase Conjugated
SKU: orb347861
Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:30,000 - 1:70,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Rat IgM whole molecule |
| Purity | Anti-Rat IgM (mu chain) was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 1.5 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit Anti-Rat IgM (mu chain) Antibody peroxidase Conjugated, Rabbit Anti-Rat IgM mu Antibody HRP Conjugation
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ELISA Results of Rabbit Anti-Rat IgM Antibody Peroxidase Conjugated tested against purified Rat IgM HRP. Each well was coated in duplicate with 1.0 µg of Rat IgM (p/n orb346341). The working dilution is 1:62800. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Rat IgM Antibody Peroxidase Conjugated (orb347861)
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