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Rat Insulin Receptor Substrate 2 (IRS2) ELISA Kit

SKU: orb780886
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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 2(IRS2).

Research Area

Cancer Biology; Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IRS2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IRS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IRS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IRS2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityRat
Sample TypesTissue homogenates and other biological fluids.
Assay TypeSandwich
Assay Time3.5h
Range0.79-50 ng/mL
Sensitivity0.32 ng/mL
Concentration50 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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Insulin receptor substrate 2, IRS 2, IRS-2, Irs2

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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