Rat Lactate Dehydrogenase A (LDHA) ELISA Kit

SKU: orb781415

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase A(LDHA).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LDHA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LDHA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.067 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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LDH-A ELISA Kit, LDH-M ELISA Kit, LDH1 ELISA Kit, PIG19 ELISA Kit, Cell proliferation-inducing gene 19 protein ELISA Kit, LDH muscle subunit ELISA Kit, Renal carcinoma antigen NY-REN-59 ELISA Kit

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Rat Lactate Dehydrogenase A (LDHA) ELISA Kit

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UniProt

P04642

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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