Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

SKU: orb776044

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha(MIP3a).

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP3a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.1 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MIP3 Alpha, CCL20 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP3-A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20 ELISA Kit, Beta-Chemokine Exodus-1 ELISA Kit, Liver And Activation-Regulated Chemokine ELISA Kit

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Rat Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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