Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) Microsample ELISA Kit

SKU: orb2997839

Description

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	24.7 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MyHC-peri; MyHC-pn; MyHC-perinatal; Myosin heavy chain, skeletal muscle, perinatal

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Rat MYH8(Myosin Heavy Chain 8, Skeletal Muscle, Perinatal) Microsample ELISA Kit

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UniProt

P04462

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No UniProt data available

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