Rat Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) ELISA Kit

SKU: orb781084

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate(MARCKS).

Research Area

Immunology & Inflammation

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MARCKS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MARCKS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MARCKS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MARCKS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.53 ng/mL
Concentration	100 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

80K-L ELISA Kit, MACS ELISA Kit, MRACKS ELISA Kit, PKCSL ELISA Kit, PRKCSL ELISA Kit, Protein kinase C substrate, 80 kDa protein, light chain ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Rat Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) ELISA Kit

Quick Database Links

UniProt

P30009

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols