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Rat PCSK9 Protein, His Tag

SKU: orb257742

Description

Rat PCSK9, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 691 (Accession # P59996-1).

Research Area

Metabolism Research

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively. The protein migrates as 16 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetPCSK9
ReactivityRat
TagC-10×His
Expression RegionGln 31 - Gln 691
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW13.9 kDa and 59.3 kDa
Purity95%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 150 mM NaCl, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only
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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP59996

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide