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Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit

SKU: orb2938715

Description

Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit

Research Area

Metabolism Research

Images & Validation

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Key Properties

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ReactivityRat
Assay TypeSandwich
Assay Time4 hours
Range1.563-100ng/ml
Sensitivity0.938ng/ml

Procedure & Performance

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Assay Principle
The kit is based on a sandwich enzyme immunoassay principle. The microtiter plate is pre-coated with a capture antibody specific to the target analyte. Standards or samples are added to the wells, followed by a biotin-conjugated detection antibody specific for the analyte. Avidin conjugated to horseradish peroxidase (HRP) is then added and incubated. After addition of the TMB substrate, color develops only in wells containing the analyte bound to the detection antibody and HRP–avidin complex. The reaction is stopped with an acidic solution, and absorbance is measured at 450 nm ± 10 nm. The analyte concentration in the samples is determined by comparison with a standard curve.
Kit Components
1. ELISA Microplate
2. Standards
3. Detection Antibody
4. HRP-Streptavidin Conjugate
5. TMB Substrate
6. Dilution buffers
7. Stop Solution
8. Wash Buffer
9. Plate Sealers
10. Manual
Reagent Preparation
1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual.
2. Standard: Perform gradient dilution according to the instructions in the manual.
3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution.
Assay Procedure
After the kit equilibrates to room temperature, add standards or samples to each well and incubate.
Discard liquid, add wash buffer to each well, wash the plate twice, and blot dry on clean absorbent paper.
Add biotinylated antibody working solution to each well and incubate.
Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper.
Add streptavidin-HRP working solution to each well and incubate.
Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper.
Add TMB substrate solution to each well and incubate in the dark.
Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm.
Materials Required
1. Microplate readers
2. Centrifuge
3. Incubator
4. Automated plate washer
5. Single-channel or multi-channel high-precision pipettes
6. Disposable pipette tips
7. Sterile tubes
8. Eppendorf tubes
9. Absorbent paper
10. Loading slots
Precision
Intra-assay Precision (Precision within an assay): CV% < 8%
Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate.

Inter-assay Precision (precision between assays): CV% < 10%
Inter-assay precision was evaluated by testing samples across different plates.
Calculation of Results
1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard.
2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points.
3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor.
Curve Fitting Softwares
1. Curve Expert
2. Thermo SkanIt RE
3. SciDAVis
4. LabPlot
5. ……

Storage & Handling

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Storage2 - 8 °C for 12 months
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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[Pyruvate dehydrogenase|acetyl-transferring)] kinase isozyme 4, mitochondrial|Pyruvate dehydrogenase kinase isoform 4|PDK4|PDHK4

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol
PDK4
UniProt
O54937

UniProt Details

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No UniProt data available

Protocol Information

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Available Sizes

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48 T
$ 370.00
96 T
$ 490.00
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