Rat Peroxiredoxin 5 (PRDX5) ELISA Kit

SKU: orb781583

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5(PRDX5).

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRDX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRDX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRDX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRDX5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	34 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PLP ELISA Kit, PRDX6 ELISA Kit, ACR1 ELISA Kit, PMP20 ELISA Kit, PRXV ELISA Kit, SBBI10 ELISA Kit, Thioredoxin Peroxidase PMP20 ELISA Kit, Peroxisomal Antioxidant Enzyme ELISA Kit, TPx Type VI ELISA Kit, Liver Tissue 2D-Page Spot 71B ELISA Kit, Alu Co-Repressor 1 ELISA Kit

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UniProt

Q9R063

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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