Rat Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

SKU: orb780369

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 11(PTPN11).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTPN11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTPN11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTPN11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTPN11 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.102 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, Noonan Syndrome 1 ELISA Kit, Protein-tyrosine phosphatase 1D ELISA Kit, Protein-tyrosine phosphatase 2C ELISA Kit

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Rat Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

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UniProt

Q06124

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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