Rat Protein Zero, Myelin (MPZ) ELISA Kit

SKU: orb778730

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Zero, Myelin(MPZ).

Research Area

Infectious Disease & Virology, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MPZ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MPZ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MPZ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MPZ in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.125 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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P0 ELISA Kit, DSS ELISA Kit, CHM ELISA Kit, CMT4E ELISA Kit, CMT1 ELISA Kit, CMT1B ELISA Kit, CMT2I ELISA Kit, CMT2J ELISA Kit, CMTDI3 ELISA Kit, HMSNIB ELISA Kit, MPP ELISA Kit, Myelin Protein Zero ELISA Kit, Charcot-Marie-Tooth Neuropathy 1B ELISA Kit, Myelin peripheral protein ELISA Kit

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Rat Protein Zero, Myelin (MPZ) ELISA Kit

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UniProt

P06907

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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