Rat Selectin, Leukocyte (SELL) ELISA Kit

SKU: orb776414

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Leukocyte(SELL).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SELL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SELL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SELL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SELL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.061 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD62L ELISA Kit, L-Selectin ELISA Kit, SEL-L ELISA Kit, LAM1 ELISA Kit, LECAM1 ELISA Kit, LNHR ELISA Kit, LSEL ELISA Kit, LYAM1 ELISA Kit, Leu-8 ELISA Kit, Lyam-1 ELISA Kit, PLNHR ELISA Kit, TQ1 ELISA Kit, HLHRc ELISA Kit, MEL14 ELISA Kit, Lymphocyte Adhesion Molecule 1 ELISA Kit, Lymph Node Homing Receptor ELISA Kit

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Rat Selectin, Leukocyte (SELL) ELISA Kit

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UniProt

P30836

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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