Rat Transgelin (TAGLN) ELISA Kit

SKU: orb780143

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transgelin(TAGLN).

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TAGLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAGLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TAGLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAGLN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.059 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SMCC ELISA Kit, SM22 ELISA Kit, TAGLN1 ELISA Kit, WS3-10 ELISA Kit, Transgelin Variant 2 ELISA Kit, 22 kDa actin-binding protein ELISA Kit, Protein WS3-10 ELISA Kit, Smooth muscle protein 22-alpha ELISA Kit

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Quick Database Links

UniProt

P31232

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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