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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | rAFP/7257 |
| Immunogen | Recombinant human full-length AFP protein |
| Target | AFP |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 70kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using rAFP/7257 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using rAFP/7257 at 2 ug/ml in PBS for 30 min RT. Inset: PBS instead of primary antibody, secondary only negative control.

SDS-PAGE Analysis of Purified AFP Recombinant Rabbit Monoclonal Antibody (rAFP/7257). Confirmation of Purity and Integrity of Antibody.
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Protocol Information
AFP Antibody (orb1410285)
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