Recombinant Coxiella burnetii Chaperonin GroEL (groEL), partial

SKU: orb1477544

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Description

This Recombinant Coxiella burnetii Chaperonin GroEL (groEL), partial spans the amino acid sequence from region 326-502aa. Purity: Greater than 85% as determined by SDS-PAGE.

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	E.coli
Biological Origin	Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212))
Tag	N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region	326-502aa
Protein Length	Partial
MW	26.4 kDa
Purity	Greater than 85% as determined by SDS-PAGE.
Protein Sequence	TKDDTTIIDGSGDAGDIKNRVEQIRKEIENSSSDYDKEKLQERLAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALIRVLKSLDSVEVENEDQRVGVEIARRAMAYPLSQIVKNTGVQAAVVADKVLNHKDVNYGYNAATGEYGDMIEMGILDPTKVTR

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Liquid or Lyophilized powder
Buffer/Preservatives	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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(60 kDa chaperonin)(Chaperonin-60)(Cpn60)

Quality Guarantee

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Quick Database Links

UniProt

B6J2I0

UniProt Details

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No UniProt data available

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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SPR (1)

P Genova-Kalou, G Dyankov, S Krumova, K Simeonov, T Valkov, M Baymakova, I Tsachev… Applicability of biosensor technologies in the detection of Coxiella burnetii infection in clinical samples Biotechnology & Biotechnological Equipment, (2024)

PubMed:

Applications

SPR

Purity:	Greater than 95% as determined by SDS-PAGE.
MW:	31.6 kDa
Source:	E.coli

Purity:	Greater than 95% as determined by SDS-PAGE.
MW:	30.8 kDa
Source:	E.coli